Reporter

Part:BBa_K5515006:Design

Designed by: Chloe William   Group: iGEM24_McMasterU   (2024-10-02)


mCherry, codon optimized for Chlamydomonas reinhardtii


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence had to be codon optimized for expression in Chlamydomonas reinhardtii.


Source

This sequence is derived from rounds of directed evolution of DsRed.

References